Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-performance immunoaffinity chromatography for drug residue analysis.

S E Katz1, M S Brady

  • 1Rutgers University, Cook College, Department of Biochemistry and Microbiology, New Brunswick, New Jersey 08903.

Journal - Association of Official Analytical Chemists
|July 1, 1990
PubMed
Summary

High-performance immunoaffinity chromatography (IAC) offers a powerful method for detecting drug residues. This paper details antibody production, column preparation, and analytical techniques for this emerging application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nodal and systemic recurrence following observation of a positive sentinel lymph node in melanoma.

The British journal of surgery·2020
Same author

Antibiotic/Antimicrobial Residues in Milk <sup>1</sup>.

Journal of food protection·2019
Same author

Cocaine-induced transmural myocardial infarction in a Yorkshire swine with normal coronary arteries: Evidence for microvascular and/or epicardial coronary artery spasm.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Thin layer chromatographic separation of linuron and diuron and some of their known or suspected soil metabolites.

Bulletin of environmental contamination and toxicology·2013
Same author

Chromatographic procedure for the determination of maloran and metabolites in soils.

Bulletin of environmental contamination and toxicology·2013
Same author

The disappearance of chlorobromuron in ensilage.

Bulletin of environmental contamination and toxicology·2013

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Immunoaffinity chromatography (IAC) is an established separation technique.
  • Its application in drug residue analysis is relatively new and developing.
  • Accurate measurement of drug residues is crucial in various fields.

Purpose of the Study:

  • To explore the potential of high-performance IAC for drug residue analysis.
  • To provide a comprehensive overview of IAC methodology for this purpose.
  • To discuss key aspects of developing and applying IAC methods for drug detection.

Main Methods:

  • Antibody production and purification strategies.
  • Immunoaffinity column preparation and characterization.
  • Theoretical principles of separation and elution in IAC.

Related Experiment Videos

  • Variations in analytical approaches for drug residue measurement.

    • Main Results:

    • Demonstration of IAC as a viable analytical tool for drug residues.
    • Detailed discussion on optimizing antibody and column parameters.
    • Explanation of the underlying chromatographic and immunological principles.
    • Presentation of adaptable analytical strategies.

    Conclusions:

    • High-performance IAC is a promising technique for sensitive drug residue quantification.
    • Further development and application of IAC methods are warranted.
    • The presented information provides a foundation for utilizing IAC in drug analysis.