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Expression profiling of Wnt signaling genes during gonadal differentiation and gametogenesis in rainbow trout
1INRA, UR1037 SCRIBE, IFR140, Ouest-Genopole, Rennes, France.
Abstract:
Wnt signaling plays major roles in various processes, including ovarian differentiation and development in mammals. In order to explore its potential implication during gonadal development in a nonmammalian vertebrate species, expression of Wnt signaling genes was investigated in rainbow trout during gonadal differentiation and gametogenesis. Multiple Wnt pathway genes were expressed and exhibited distinct expression patterns. In ovary, tcf7 was highly expressed during early differentiation, whereas no sexually dimorphic expression of rspo1 was detected. During later ovarian development, wnt11 was highly expressed in granulosa cells and oocytes suggesting an implication in folliculogenesis and oogenesis, whereas wnt9b was principally detected in granulosa cells. In testis, Wnt pathway genes were mostly expressed during early spermatogenesis. Overall, these present results suggest that Wnt signaling is implicated in multiple processes of male and female gonadal development and provide basis for future studies on Wnt signaling functions in teleost fish gonads.
Insights
Wnt signaling is crucial for fish gonad development. This study reveals distinct Wnt gene expression patterns in rainbow trout ovaries and testes, highlighting its role in both male and female reproductive processes.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Fish Biology
Background:
- Wnt signaling is essential for mammalian ovarian differentiation and development.
- Its role in nonmammalian vertebrate gonadal development remains less understood.
- Rainbow trout (Oncorhynchus mykiss) serves as a model for teleost fish reproduction.
Purpose of the Study:
- To investigate the expression patterns of Wnt signaling pathway genes during gonadal differentiation and gametogenesis in rainbow trout.
- To explore the potential involvement of Wnt signaling in the reproductive development of a teleost species.
- To identify sexually dimorphic and stage-specific expression of Wnt genes in gonads.
Main Methods:
- Gene expression analysis of multiple Wnt pathway genes in rainbow trout gonads.
- Investigation across different stages of gonadal differentiation and gametogenesis.
- Comparative analysis of gene expression in developing ovaries and testes.
Main Results:
- Multiple Wnt pathway genes were expressed in rainbow trout gonads with distinct patterns.
- In the ovary, tcf7 showed high expression during early differentiation; wnt11 was prominent in granulosa cells and oocytes during later development.
- Wnt pathway genes were predominantly expressed during early spermatogenesis in the testis.
Conclusions:
- Wnt signaling is implicated in various processes of both male and female gonadal development in rainbow trout.
- The findings provide a foundation for future research into specific Wnt signaling functions in teleost fish gonads.
- Distinct Wnt gene expression suggests conserved and divergent roles in vertebrate reproductive biology.
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