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Cell migration does not produce membrane flow.

D F Kucik1, E L Elson, M P Sheetz

  • 1Department of Cell Biology, Washington University School of Medicine, St. Louis, Missouri 63110.

The Journal of Cell Biology
|October 1, 1990
PubMed
Summary
This summary is machine-generated.

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Cell membrane flow does not drive particle migration. Single particle tracking on fish keratocytes revealed no bulk membrane lipid flow, even at high cell speeds.

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Previous studies ruled out rapid rearward membrane flow and particle migration driven by membrane flow in slowly moving cells.
  • The possibility of slower, tank-tread membrane lipid flow remained unaddressed.

Purpose of the Study:

  • To investigate membrane lipid flow in rapidly locomoting cells.
  • To determine if cytoskeleton-driven protein motion or other factors cause bulk membrane lipid flow.

Main Methods:

  • Utilized single particle tracking technology.
  • Observed Con A-coated gold particles on the surface of rapidly moving fish keratocytes.

Main Results:

  • Particles exhibited random diffusion (apparent diffusion coefficient of [3.5 +/- 2.0] x 10(-10) cm2/s) or slow rearward tracking (0.35 +/- 0.15 micron/s).

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  • No systematic drift of particles relative to the cell edge was detected, even after significant cell movement.
  • The average net particle displacement was negligible (0.01 +/- 2.7%) compared to cell displacement.

    • Conclusions:

    • The findings strongly suggest the absence of bulk membrane lipid flow in fish keratocytes.
    • Neither cytoskeleton-driven membrane protein dynamics nor other factors appear to generate significant membrane flow.