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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
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Related Experiment Video

Updated: May 27, 2026

MISSION esiRNA for RNAi Screening in Mammalian Cells

MISSION esiRNA for RNAi Screening in Mammalian Cells

Published on: May 12, 2010

RNAi methods and screening: RNAi based high-throughput genetic interaction screening.

Patricia G Cipriani¹, Fabio Piano

¹Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10003, USA.

Methods in Cell Biology

|November 29, 2011

Summary

This summary is machine-generated.

High-throughput RNA interference (RNAi) screens in C. elegans enable the discovery of gene functions. This method facilitates systematic testing of gene interactions and condition-dependent effects for enhanced genetic analysis.

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Last Updated: May 27, 2026

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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

Published on: February 27, 2012

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Decades of mutational analyses and genome-scale RNAi screens in C. elegans suggest most essential genes have been identified.
Uncovering condition-dependent or combinatorial gene effects requires higher-scale screening methods.

Purpose of the Study:

To describe a high-throughput RNAi screening method in C. elegans using 96-well liquid plates.
To detail protocols for systematically testing gene enhancement and suppression of temperature-sensitive mutations.

Main Methods:

High-throughput RNAi screening in liquid C. elegans cultures within 96-well plates.
Systematic testing for enhancement and suppression of temperature-sensitive mutations.
Rapid acquisition of high-quality images for quantitative scoring and computerized image analysis.

Main Results:

The described method allows for efficient management of numerous samples per screening cycle.
Enables quantitative scoring and computerized image analysis for large-scale screening.
Facilitates result review independent of time constraints associated with extensive screening.

Conclusions:

This high-throughput RNAi screening approach advances C. elegans research by enabling deeper investigation of gene function.
The method supports the discovery of condition-dependent and combinatorial gene effects, expanding beyond essential gene identification.
Optimized imaging and analysis protocols enhance the efficiency and scope of large-scale genetic screens.