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Related Experiment Video

Updated: May 27, 2026

Generation of Stable Transgenic C. elegans Using Microinjection
12:09

Generation of Stable Transgenic C. elegans Using Microinjection

Published on: August 15, 2008

Transgenesis in C. elegans.

Vida Praitis1, Morris F Maduro

  • 1Biology Department, Grinnell College, Grinnell, Iowa, USA.

Methods in Cell Biology
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

Genome manipulation in the nematode Caenorhabditis elegans has advanced significantly since the 1980s. This review covers methods for creating transgenics, their applications, and future directions for C. elegans and other nematodes.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Genetic manipulation is crucial for understanding biological systems.
  • Transgenic techniques in Caenorhabditis elegans emerged in the late 1980s.
  • Subsequent advancements have refined transgene expression and targeting.

Purpose of the Study:

  • To review current methods for generating transgenics in C. elegans.
  • To discuss the applications and limitations of various transgenic approaches.
  • To explore future developments in nematode transgenesis.

Main Methods:

  • Review of established and novel protocols for C. elegans transgenesis.
  • Comparative analysis of different transgene expression and chromosomal targeting strategies.

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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Published on: December 9, 2013

Generation of Transgenic C. elegans by Biolistic Transformation
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Generation of Transgenic C. elegans by Biolistic Transformation

Published on: August 23, 2010

Related Experiment Videos

Last Updated: May 27, 2026

Generation of Stable Transgenic C. elegans Using Microinjection
12:09

Generation of Stable Transgenic C. elegans Using Microinjection

Published on: August 15, 2008

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
06:33

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Published on: December 9, 2013

Generation of Transgenic C. elegans by Biolistic Transformation
10:15

Generation of Transgenic C. elegans by Biolistic Transformation

Published on: August 23, 2010

  • Discussion of applications in biological research.

    • Main Results:

    • Significant improvements in transgene expression efficiency and control.
    • Development of methods for targeted transgene integration.
    • Expanded utility of transgenes for diverse biological inquiries.

    Conclusions:

    • C. elegans transgenesis is a powerful tool for biological research.
    • Ongoing innovations continue to enhance the capabilities of transgenic nematodes.
    • These methods hold promise for application in other nematode species.