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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: May 27, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Analysis of microRNA expression and function.

Priscilla M Van Wynsberghe1, Shih-Peng Chan2, Frank J Slack2

  • 1Department of Biology, University of California San Diego, La Jolla, California, USA.

Methods in Cell Biology
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) are small RNAs regulating cellular processes. This review covers methods in C. elegans for studying miRNA biogenesis, target identification, and gene regulation.

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Related Experiment Videos

Last Updated: May 27, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Published on: March 30, 2019

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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • MicroRNAs (miRNAs) are endogenous small RNA molecules that play crucial roles in post-transcriptional gene regulation.
  • They are involved in fundamental cellular processes across diverse organisms, from development to disease.
  • The study of miRNAs has expanded significantly due to their conserved nature and importance in biological pathways.

Purpose of the Study:

  • To review current methodologies for investigating miRNA biology.
  • Focus on techniques applicable in the model organism Caenorhabditis elegans.
  • To provide insights into miRNA biogenesis, target identification, and regulatory mechanisms.

Main Methods:

  • Analysis of miRNA biogenesis pathways, including Drosha and Dicer processing.
  • Methods for characterizing small RNA populations and their abundance.
  • Techniques for identifying miRNA targets and studying miRNA-protein interactions within the RNA-induced silencing complex (RISC).

Main Results:

  • C. elegans serves as a powerful model organism for advancing miRNA research.
  • Established methods allow for detailed investigation of miRNA processing and function.
  • Advances in target identification and regulatory mechanism studies are highlighted.

Conclusions:

  • Caenorhabditis elegans provides a robust platform for dissecting complex miRNA pathways.
  • Current methodologies enable comprehensive analysis of miRNA biogenesis and function.
  • Further research in C. elegans will continue to elucidate miRNA roles in gene regulation and cellular processes.