Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phospholipid biogenesis maintains neuronal integrity during aging and axon regeneration.

Genetics·2025
Same author

Multiscale patterning of a model apical extracellular matrix revealed by systematic endogenous protein tagging.

bioRxiv : the preprint server for biology·2025
Same author

A defining member of the new cysteine-cradle family is an aECM protein signalling skin damage in C. elegans.

PLoS genetics·2025
Same author

Context-specific interaction of the lipid regulator DIP-2 with phospholipid synthesis in axon regeneration and maintenance.

bioRxiv : the preprint server for biology·2025
Same author

The BEN domain protein LIN-14 coordinates neuromuscular positioning during epidermal maturation.

iScience·2025
Same author

A defining member of the new cysteine-cradle family is an aECM protein signalling skin damage in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2024
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
06:30

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

Cell identification and cell lineage analysis.

Claudiu A Giurumescu1, Andrew D Chisholm

  • 1Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California, USA.

Methods in Cell Biology
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

Caenorhabditis elegans offers a powerful model for studying cell development. Its transparent body and invariant cell positions allow detailed tracking of cell lineages, crucial for understanding gene function in development.

More Related Videos

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
07:48

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Published on: May 8, 2020

Related Experiment Videos

Last Updated: May 27, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
06:30

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM

Published on: March 2, 2017

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
07:48

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Published on: May 8, 2020

Area of Science:

  • Developmental Biology
  • Genetics
  • Cell Biology

Background:

  • Caenorhabditis elegans possesses a simple, invariant somatic cell count and predictable cell positioning.
  • Its near-complete transparency facilitates cellular observation in vivo.

Purpose of the Study:

  • To highlight the utility of Caenorhabditis elegans as a model organism for detailed cell lineage analysis.
  • To underscore the importance of cell lineage studies in defining developmental gene functions.

Main Methods:

  • Utilizing bright-field microscopy, Nomarski differential interference contrast (DIC), and transgenic fluorescent reporter genes for cell identification.
  • Employing timelapse imaging for continuous observation of embryonic and postembryonic development.
  • Semiautomated tracing of embryonic cell lineages using GFP-labeled nuclei.

Main Results:

  • Demonstrated the feasibility of tracking cell positions and morphology with high fidelity in live C. elegans.
  • Successfully analyzed cell lineages throughout both embryonic and postembryonic developmental stages.
  • Facilitated the identification of developmental control genes through mutant cell lineage analysis.

Conclusions:

  • Caenorhabditis elegans is an exceptional model for comprehensive cell lineage mapping.
  • Cell lineage analysis in C. elegans is vital for elucidating gene function in developmental processes.
  • The described methods enable precise tracking of cell fate and development.