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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 27, 2026

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
09:59

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Published on: February 12, 2014

Volume electron microscopy for neuronal circuit reconstruction.

Kevin L Briggman1, Davi D Bock

  • 1Circuit Dynamics and Connectivity Unit, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA. briggmankl@mail.nih.gov

Current Opinion in Neurobiology
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

New electron microscopy (EM) tools accelerate neural circuit mapping. Volume EM imaging, while crucial for neuronal

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A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
12:49

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

Related Experiment Videos

Last Updated: May 27, 2026

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
09:59

Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Published on: February 12, 2014

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
12:49

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

Area of Science:

  • Neuroscience
  • Microscopy
  • Computational Biology

Background:

  • Recent advancements have significantly increased the availability of tools for acquiring volume electron microscopy (EM) data.
  • Emerging scanning electron microscopy (SEM) techniques and scaled-up transmission electron microscopy (TEM) methods are transforming data acquisition.

Purpose of the Study:

  • To review and compare novel methods for large-volume EM data acquisition.
  • To evaluate the suitability of these methods for reconstructing neuronal anatomical connectivity.
  • To discuss the future potential and limitations of volume EM in neuroscience.

Main Methods:

  • Summary of new volume EM acquisition techniques.
  • Analysis of tradeoffs: resolution, speed, and reliability.
  • Assessment of applicability for neuronal connectomics.

Main Results:

  • Several new SEM and automated TEM methods are available for large-volume EM data acquisition.
  • Tradeoffs in resolution, speed, and reliability vary among methods.
  • Current capabilities and future prospects for neuronal connectivity reconstruction are assessed.

Conclusions:

  • Neuronal 'wiring diagrams' are essential but insufficient for understanding neural circuit function.
  • Volume EM is most powerful when integrated with other neuroscience techniques.
  • Future applications include combining EM with molecular biology, optogenetics, and physiology.