Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cyclic nucleotide-dependent protein kinase modulators in thyroid tissue.

K Hashizume, L J DeGroot

    Endocrinology
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Newly identified interferon tau-responsive Hes family BHLH transcription factor 4 and cytidine/uridine monophosphate kinase 2 genes in peripheral blood granulocytes during early pregnancy in cows.

    Domestic animal endocrinology·2019
    Same author

    Suppression of Common Scab of Potato Caused by Streptomyces turgidiscabies Using Lopsided Oat Green Manure.

    Plant disease·2019
    Same author

    Mid-term results of alumina ceramic unlinked total elbow arthroplasty with cement fixation for patients with rheumatoid arthritis.

    The bone & joint journal·2018
    Same author

    Evaluation of interferon-stimulated genes in peripheral blood granulocytes as sensitive responders to bovine early conceptus signals.

    Veterinary journal (London, England : 1997)·2017
    Same author

    POU3F2 participates in cognitive function and adult hippocampal neurogenesis via mammalian-characteristic amino acid repeats.

    Genes, brain, and behavior·2017
    Same author

    Biogenesis of Protein Bodies in Embryonic Axes of Soybean Seeds (Glycine max. cv. Enrei).

    Bioscience, biotechnology, and biochemistry·2016
    Same journal

    Zingerone ameliorates ovarian impairment by regulating steroidogenesis and apoptosis in letrozole-treated mouse.

    Endocrinology·2026
    Same journal

    Loss of the calorie restriction response protein DEPP1 worsens diet-induced obesity.

    Endocrinology·2026
    Same journal

    Long Non-Coding RNAs Mediate Endocrine Signaling and Resistance in Prostate Cancer.

    Endocrinology·2026
    Same journal

    Repeated Chemogenetic Activation of C1 Catecholamine Neurons Reduces Subsequent Glucoprivic Responses and Mimics HAAF.

    Endocrinology·2026
    Same journal

    Postnatal exposure to maternal hypothyroidism leads to developmental delay and metabolic dysregulations in male mice.

    Endocrinology·2026
    Same journal

    Discovery proteomics identification of factors contributing to gonadotropin β expression.

    Endocrinology·2026
    See all related articles

    Researchers isolated a heat-stable protein that inhibits cyclic adenosine monophosphate (cAMP) dependent protein kinase in bovine thyroid. Another modulator stimulated cyclic guanosine monophosphate (cGMP) dependent protein kinase, suggesting distinct roles in thyroid function.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Endocrinology

    Background:

    • Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are crucial second messengers in cellular signaling.
    • Protein kinases play vital roles in regulating cellular processes through phosphorylation.
    • Understanding the regulation of these kinases is essential for comprehending thyroid function.

    Purpose of the Study:

    • To isolate and characterize modulators of cAMP-dependent protein kinase (PKA) and cGMP-dependent protein kinase (PKG) from bovine thyroid.
    • To investigate the distinct roles of these modulators in thyroid cell signaling pathways.

    Main Methods:

    • Preparation of a heat-stable inhibitory protein from bovine thyroid 100,000 X g supernatant.
    • Separation of a stimulatory modulator for cGMP-dependent protein kinase using Sephadex G-100 gel filtration.

    Related Experiment Videos

  • Assays to determine the effects of isolated modulators on cAMP- and cGMP-dependent protein kinase activities.

    • Main Results:

    • A heat-stable protein inhibiting both cytosolic and plasma membrane cAMP-dependent protein kinase was isolated.
    • The inhibitory effect on cytosolic cAMP-dependent protein kinase was noncompetitive with histone.
    • A distinct stimulatory modulator for cGMP-dependent protein kinase was identified and separated.
    • The identified modulators exhibited specificity, with no cross-activity between cAMP and cGMP systems.

    Conclusions:

    • The inhibitory modulator likely plays a role in regulating cAMP-dependent protein kinase activity within the bovine thyroid cytosol and plasma membrane.
    • The stimulatory modulator for cGMP-dependent protein kinase may have an independent role in thyroid function, separate from the cAMP signaling pathway.