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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: May 27, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

[RNA-Seq and its applications: a new technology for transcriptomics].

Yun-Xia Qi1, Yong-Bin Liu, Wei-Heng Rong

  • 1College of Animals Science, Inner Mongolia Agriculture University, Huhhot, China. qi_yunxia@163.com

Yi Chuan = Hereditas
|November 29, 2011
PubMed
Summary
This summary is machine-generated.

RNA sequencing (RNA-seq) offers a comprehensive view of cellular transcripts, aiding in understanding biological processes and diseases. This review details RNA-seq principles, applications, and future potential for researchers.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • The transcriptome represents the complete set of RNA transcripts in cells or tissues.
  • Understanding gene structure and function via transcriptome analysis is crucial for deciphering biological mechanisms and diseases.
  • Transcriptome analysis has been significantly advanced by the development of RNA sequencing (RNA-seq).

Purpose of the Study:

  • To review the principles, technical characteristics, and applications of RNA sequencing (RNA-seq).
  • To discuss the challenges and future potential of RNA-seq in biological, medical, clinical, and pharmaceutical research.

Main Methods:

  • RNA sequencing (RNA-seq) utilizes high-throughput sequencing to analyze RNA transcripts.
  • RNA-seq involves sequencing cDNA libraries derived from cellular or tissue RNA.
  • Transcripts are mapped to a reference genome to obtain genetic information, including transcription localization and alternative splicing.

Main Results:

  • RNA-seq enables quantification, profiling, and discovery of RNA transcripts.
  • It provides comprehensive genetic information such as transcription localization and alternative splicing status.
  • RNA-seq is widely applied across biological, medical, clinical, and pharmaceutical research fields.

Conclusions:

  • RNA-seq is a powerful tool for transcriptome analysis, offering deep insights into molecular mechanisms.
  • The technology presents ongoing challenges but holds significant potential for future research applications.
  • This review provides valuable information on RNA-seq for the scientific community.