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Published on: November 5, 2019
Decoding cell lineage from acquired mutations using arbitrary deep sequencing
Cheryl A Carlson1, Arnold Kas, Robert Kirkwood
1Department of Pathology, University of Washington School of Medicine, Seattle, Washington, USA.
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Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.
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