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[Component difference of herb materials extracts with sesame oil fry and SFE-CO2 technique for compound ulcer oil]
Jing Li1, Bohua Yang, Lijuan Yang
1School of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100102, China.
Objective:
To compare the component difference of herb materials extracts of sesame oil fry and SFE-CO2 technique for compound ulcer oil.
Method:
Qualitative analysis of main component of dahuan, baizhi and chuangxiong in two extracts above was conducted by TLC. The contents of total anthraquinones, imperatorin and ferulic acid in two extracts were determined by UV and HPLC.
Result:
TLC experiment found that spots color of small Rf value component in oil extract were lighter than that in SFE-CO2 extract, but there was not obvious different between two extracts. Quantity analysis showed that SFE-CO2 extract owned much higher transfer rate of total anthraquinones, and it was 1.9 times of oil extract. Ferulic acid was similar in two extracts, and they were all below 10%. The contents of imperatorin in oil extracts were slight higher than that in SFE-CO2 extract.
Conclusion:
The components in the extracts of sesame oil fry for the herb materials of compound ulcer oil are the same as SFE-CO2 extract. Because SFE-CO2 extracts have no solvent limited for next preparation, it has more advantage.