Related Experiment Video
Updated: May 27, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
Published on: March 12, 2021
Epigenome sequencing comes of age in development, differentiation and disease mechanism research
1School of Life Science & Technology, Tongji University, 1239 Siping Road, Shanghai 200092, China.
Abstract:
In eukaryotic organisms, changes in cell phenotype are tightly associated with dynamic changes in the epigenome. Over the past few years, sequencing-based genome-wide approaches to generate, analyze, interpret and integrate epigenetic information have been applied to investigate the mechanisms behind the changes in cell status, such as those which are seen in differentiation, disease and reprogramming. This article focuses on the four types of epigenomic information (i.e., nucleosome positioning, histone modification, DNA methylation and chromatin higher-order structure). We summarize the distinct high-throughput sequencing applications used to generate the different types of epigenomic profiles and the bioinformatic software available for performing routine analysis. With the dramatic improvement of sequencing technology and bioinformatic analysis, epigenome sequencing has gradually become the common approach to study a variety of biological issues.
More Related Videos
Related Concept Videos
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genomics
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing
Evolutionary Relationships through Genome Comparisons
Modern Molecular Taxonomy

