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Updated: May 27, 2026
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A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status
Published on: October 20, 2016
Age-dependent changes in the cerebrospinal fluid proteome by slow off-rate modified aptamer array.
Geoffrey S Baird1, Sally K Nelson, Tracy R Keeney
1Department of Laboratory Medicine, University of Washington, Harborview Medical Center, Box 359743, 325 9th Avenue, Seattle, WA 98105, USA. gbaird@u.washington.edu
The American Journal of Pathology
|November 30, 2011
Summary
Understanding cerebrospinal fluid (CSF) protein changes during aging is key for neurodegenerative disease diagnostics. This study used novel proteomic technology to identify age-related protein shifts in normal aging adults.
Area of Science:
- Neuroscience
- Proteomics
- Gerontology
Background:
- Cerebrospinal fluid (CSF) proteome dynamics during normal aging are crucial for developing diagnostic assays for age-related neurodegenerative diseases.
- Understanding these changes is a critical step towards identifying reliable biomarkers.
Purpose of the Study:
- To utilize a novel proteomic technology to quantify hundreds of proteins simultaneously in the CSF of cognitively normal adults across a wide age range.
- To investigate the dynamic changes in the CSF proteome associated with the normal aging process.
Main Methods:
- Employed SomaLogic's highly multiplexed proteomic platform utilizing slow off-rate modified aptamer (SOMAmer) reagents.
- Analyzed CSF samples from 90 cognitively normal individuals aged 21 to 85 years.
- Quantified over 800 proteins per sample with high sensitivity and specificity.
Main Results:
- Successfully detected 248 proteins in the CSF with signals exceeding twofold over background.
- Discovered novel correlations between specific protein concentrations and chronological age.
- Identified potential age-associated increases in central nervous system inflammation and response to injury markers.
Conclusions:
- This novel proteomic approach provides significant new insights into the aging of the human central nervous system.
- The findings suggest potential utility in discovering new disease-related changes within the CSF proteome for diagnostic purposes.
- Highlights the importance of studying CSF proteomic profiles in aging for future biomarker discovery.
