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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
IL-17A and IL-17F expression in B lymphocytes.
Alejandro Vazquez-Tello1, Rabih Halwani, Rui Li
1Asthma Research Chair, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
International Archives of Allergy and Immunology
|November 30, 2011
Summary
Human B lymphocytes can produce interleukin-17A (IL-17A) and interleukin-17F (IL-17F) cytokines. These findings suggest B cells play a significant role in immune responses beyond antibody production.
Area of Science:
- Immunology
- Cell Biology
Background:
- Th-17 lymphocytes are not the sole producers of interleukin (IL)-17A and IL-17F.
- B lymphocytes are recognized cytokine producers in chronic inflammatory diseases.
Purpose of the Study:
- To investigate the capacity of human B lymphocytes to produce IL-17A and IL-17F.
- To determine if B cells contribute to IL-17A and IL-17F production in immune-mediated diseases.
Main Methods:
- Purified human B cells (CD19+) were stimulated with various cytokines (IL-4, interferon-γ, IL-6, IL-23, TGF-β).
- IL-17A and IL-17F expression was quantified using RT-PCR, Western blot, immunocytochemistry, and ELISA.
Main Results:
- Evidence of IL-17A and IL-17F expression was confirmed in purified B cells.
- Stimulation with IL-6, IL-23, and TGF-β significantly upregulated IL-17A and IL-17F production.
Conclusions:
- Cytokine-stimulated B lymphocytes are a significant source of IL-17A and IL-17F.
- B cells actively contribute to immune responses through mechanisms beyond antibody secretion.
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