Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

What is Gene Expression?

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Density functional theory investigation of adsorption and charge transfer of fungal polyketide mycotoxins on Si, N Co-doped carbon quantum dots.

Journal of molecular modeling·2026

Same author

The long noncoding RNA <i>lnc-FAM164A1</i>-ACLY axis promotes pro-inflammatory responses in human primary macrophages: a systems approach.

Frontiers in immunology·2026

Same author

DoTools: a cross platform framework to streamline common single cell workflows.

Bioinformatics advances·2026

Same author

Risk factors for prolonged hospital stay after surgery in older patients.

Anaesthesia and intensive care·2026

Same author

Associations Between Transsphenoidal Surgery and Neuropsychiatric Disorders for Patients With Cushing's Disease.

Neurosurgery·2026

Same author

Stereotactic radiosurgery for pineal gland metastases: results from the international radiosurgery research foundation.

Journal of neuro-oncology·2026

Search research articles

Related Experiment Video

Updated: May 27, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Gene Array Analyzer: alternative usage of gene arrays to study alternative splicing events.

Pascal Gellert¹, Mizue Teranishi, Katharina Jenniches

¹Max-Planck Institute for Heart and Lung Research, Ludwigstrasse 43, 61231 Bad Nauheim, Germany.

Nucleic Acids Research

|November 30, 2011

Summary

Gene Array Analyzer (GAA) enables affordable alternative splicing analysis using gene arrays, expanding transcriptomic insights. This method revealed developmental splicing events active in both embryonic heart development and disease states.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: May 27, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Exon arrays are standard for differential splicing analysis.
Gene arrays primarily measure transcript expression but share design similarities with exon arrays.

Purpose of the Study:

To introduce the Gene Array Analyzer (GAA) for economic alternative splicing analysis using gene arrays.
To demonstrate GAA's utility by profiling splicing during embryonic heart development.

Main Methods:

Development of the Gene Array Analyzer (GAA) from the Exon Array Analyzer (EAA).
Application of GAA to analyze alternative splicing events in embryonic heart development.
Comparison of gene arrays and exon arrays for splicing analysis.

Main Results:

GAA provides an economic and user-friendly method for splicing analysis on gene arrays.
Numerous developmental splicing events were identified during embryonic heart development.
These developmental splicing events were also found to be active under pathological conditions.

Conclusions:

GAA significantly expands the capabilities of gene arrays for expression analysis, adding splicing information.
Utilizing GAA offers additional transcriptomic data with minimal cost and effort.
The findings highlight the link between developmental and pathological splicing events.