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Updated: May 27, 2026

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
Published on: May 28, 2021
The C. elegans DAF-2 insulin-like receptor is abundantly expressed in the nervous system and regulated by nutritional
K D Kimura1, D L Riddle, G Ruvkun
1Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
Abstract:
A Caenorhabditis elegans insulin-like signaling pathway regulates development, metabolism, and longevity. We detected abundant DAF-2 insulin-like receptor protein mainly in the nervous system, consistent with the assignment of DAF-2 pathway regulation of longevity to the nervous system. DAF-2 abundance in the nervous system is dependent on food intake, showing environmental modulation of pathway signaling. DAF-2 abundance is not dependent on downstream PI-3 kinase to DAF-16 transcription factor signaling. The modulation of DAF-2 protein level by nutritional status may constitute an important component in the irreversible commitment to dauer arrest.
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