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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Updated: May 27, 2026

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Getting the whole picture: combining throughput with content in microscopy.

Nitzan Rimon1, Maya Schuldiner

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel 76100.

Journal of Cell Science
|November 30, 2011
PubMed
Summary

High-content screening (HCS) revolutionizes biological sciences by enabling systematic, single-cell data acquisition. This high-throughput microscopy approach combines data volume with biological depth for advanced cell biology research.

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Last Updated: May 27, 2026

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
12:32

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14:09

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Area of Science:

  • Cell Biology
  • Biological Sciences
  • High-Throughput Science

Background:

  • Automated scientific equipment has surged, driving a revolution in biological sciences.
  • High-throughput science enables new biological questions through systematic, unbiased data generation.
  • High-content screening (HCS) facilitates systematic, single-cell level data acquisition.

Purpose of the Study:

  • To discuss recent work demonstrating the diverse applications of HCS.
  • To highlight evolving technological solutions in the HCS field.
  • To showcase how HCS combines throughput with biological content for cell biology research.

Main Methods:

  • High-throughput microscopy (high-content screening) for systematic data acquisition.
  • Visualization of a wide array of cellular features.
  • Quantification of numerous parameters per cell.

Main Results:

  • HCS generates biologically meaningful, rich data without sacrificing systematic capabilities.
  • Recent studies showcase the versatility and expanding applications of HCS.
  • Technological advancements are pushing HCS methodologies to the forefront of high-throughput science.

Conclusions:

  • HCS is a powerful method for addressing complex cell biological questions.
  • The integration of throughput and content in HCS is crucial for modern biological research.
  • Ongoing advancements position HCS as a key technology for future biological discoveries.