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Related Experiment Video

Updated: May 27, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Prohibitin has an important role in adipocyte differentiation.

S R Ande1, Z Xu, Y Gu

  • 1Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.

International Journal of Obesity (2005)
|November 30, 2011
PubMed
Summary

Prohibitin (PHB) is upregulated by insulin and leptin, playing a key role in adipocyte differentiation. Overexpressing PHB in preadipocytes can initiate the fat cell development process.

Related Experiment Videos

Last Updated: May 27, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Metabolism

Background:

  • Adipocyte differentiation is a complex process regulated by various signaling pathways.
  • The role of prohibitin (PHB) in adipogenesis is not fully understood.

Purpose of the Study:

  • To investigate if prohibitin (PHB) is a target gene in adipocyte differentiation.
  • To determine if PHB modulates insulin-induced adipocyte differentiation.

Main Methods:

  • 3T3-L1 preadipocytes were engineered to overexpress wild-type PHB or a PHB mutant lacking tyrosine-114 phosphorylation.
  • Cells were treated with insulin or a peroxisome proliferator-activated receptor-γ agonist.
  • Reporter gene assays were used to analyze PHB promoter activity.

Main Results:

  • Insulin and PPARγ agonist treatment upregulated PHB in a dose- and time-dependent manner.
  • PHB promoter contains insulin-response and C/EBP elements, suggesting transcriptional regulation.
  • PHB overexpression alone was sufficient to induce adipogenesis in 3T3-L1 fibroblasts.
  • Leptin was also identified as a regulator of PHB.

Conclusions:

  • Prohibitin (PHB) is identified as a crucial protein in adipocyte differentiation.
  • PHB plays a significant role in insulin-mediated adipogenesis.
  • PHB is a novel regulator of fat cell development.