Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 27, 2026

Generating Genetically Modified Plasmodium berghei Sporozoites
10:16

Generating Genetically Modified Plasmodium berghei Sporozoites

Published on: May 5, 2023

Progress with schistosome transgenesis.

Yousef Noori Alrefaei1, Tunika Ida Okatcha, Danielle Elaine Skinner

  • 1Department of Microbiology, Immunology and Tropical Medicine, George Washington University Medical Center, Washington, DC, USA.

Memorias Do Instituto Oswaldo Cruz
|November 30, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Chromosome level genome assembly of the World Health standards Leishmania (Viannia) guyanensis M4147 and L. (V.) shawi M8408 using a hybrid sequencing approachFIRST REVIEW ROUND - REVIEWERS' COMMENTSREVIEWERS' COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Uptake and survival of Leishmania amazonensis in Acanthamoeba: an adequate model organism?FIRST REVIEW ROUND - REVIEWERS' COMMENTSREVIEWERS' COMMENTSREVIEWERS' COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Dengue virus serotype 4 infection in human fatal cases: histopathological investigationFIRST REVIEW ROUND - REVIEWERS COMMENTSREVIEWERS COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Novel epitope-based diagnostic probes selected by phage display for the serological detection of HDV

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Coexistence of monogamy and polygyny in Triatoma infestans: fine-scale genealogical structure reveals complex social structures within domestic colonies in the Bolivian Chaco

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Is Wolbachia a sustainable component for dengue control?FIRST REVIEW ROUND - REVIEWERS COMMENTSREVIEWERS COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026

Transgenesis tools enable functional genomics in schistosomes. Researchers developed methods using retroviruses and transposons for gene manipulation, aiding in understanding schistosome biology.

Area of Science:

  • Parasitology
  • Genomics
  • Molecular Biology

Background:

  • Genome sequences for Schistosoma japonicum and Schistosoma mansoni are available.
  • Understanding the function of schistosome genes is crucial for developing treatments.
  • Transgenesis offers powerful tools for functional genomic studies in schistosomes.

Purpose of the Study:

  • To develop and evaluate tools for transgenesis in schistosomes.
  • To facilitate gain-of-function and loss-of-function studies in schistosome gene sequences.

Main Methods:

  • Investigated retroviruses, specifically vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped murine leukemia virus (MLV), for schistosome transduction.
  • Assessed the utility of the piggyBac transposon for transpositional activity in schistosomes.

More Related Videos

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Published on: August 16, 2011

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
09:42

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Published on: October 7, 2021

Related Experiment Videos

Last Updated: May 27, 2026

Generating Genetically Modified Plasmodium berghei Sporozoites
10:16

Generating Genetically Modified Plasmodium berghei Sporozoites

Published on: May 5, 2023

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
05:30

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Published on: August 16, 2011

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
09:42

Generating Transgenics and Knockouts in Strongyloides Species by Microinjection

Published on: October 7, 2021

  • Utilized reporter transgenes to confirm somatic transgenesis and chromosomal integration.

    • Main Results:

    • VSVG-MLV successfully transduced developmental stages of Schistosoma mansoni, including eggs.
    • The piggyBac transposon demonstrated transpositional activity within schistosomes.
    • Both VSVG-MLV and piggyBac approaches led to somatic transgenesis and integration of active reporter transgenes into schistosome chromosomes, marking the first reports of such integration.

    Conclusions:

    • Developed and validated novel tools for schistosome transgenesis.
    • These tools enable functional genomic investigations, including insertional mutagenesis and RNA interference (RNAi).
    • Findings pave the way for advanced gene manipulation studies in schistosomes, including germ line transgenesis.