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Detection and Isolation of Campylobacter spp. from Raw Meat
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Pathogenic potential of Campylobacter ureolyticus.

Jose A Burgos-Portugal1, Nadeem O Kaakoush, Mark J Raftery

  • 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia.

Infection and Immunity
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Campylobacter ureolyticus adheres to intestinal cells and causes damage, suggesting it may be a gastrointestinal pathogen. Further research identified potential virulence factors in its secretome.

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Pathogen Discovery

Background:

  • Campylobacter ureolyticus, formerly Bacteroides ureolyticus, was recently found in children with Crohn's disease.
  • The pathogenic potential of this bacterium in the gastrointestinal tract requires investigation.

Purpose of the Study:

  • To assess the pathogenic potential of Campylobacter ureolyticus.
  • To investigate its adherence, invasion, and interaction with intestinal cells.

Main Methods:

  • Utilized adherence and gentamicin protection assays with Caco-2 cells.
  • Employed scanning electron microscopy to visualize bacterial-host cell interactions.
  • Analyzed the C. ureolyticus secretome for potential virulence factors.

Main Results:

  • C. ureolyticus adhered to Caco-2 cells (5.341% ± 0.74%) but did not invade.
  • Proinflammatory cytokines (TNF-α, IFN-γ) did not significantly alter adherence or invasion.
  • Microscopy showed C. ureolyticus colonization, cellular damage, and microvillus degradation.
  • Identified 29 putative secretory proteins, including CjaA, fibronectin binding protein, and S-layer RTX toxin.

Conclusions:

  • C. ureolyticus demonstrates adherence and colonization capabilities in the intestinal tract.
  • The bacterium possesses potential virulence factors, suggesting a role in gastrointestinal pathogenesis.
  • Further research is warranted to elucidate the specific mechanisms of C. ureolyticus pathogenicity.