Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Size-Exclusion Chromatography

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multiple Heart-Cut Ion-Exchange Chromatography-Reversed-Phase Liquid Chromatography Platform for Online Desalting and Fractionation of Monoclonal Antibody Charge Variants.

Journal of separation science·2026

Same author

Enabling Large-Volume Injections in Hydrophilic Interaction Chromatography of Oligonucleotides Through In-Line Mixing.

Journal of separation science·2026

Same author

Accelerating microfluidic capillary electrophoresis-mass spectrometry for charge-variant and glycoform analysis of intact monoclonal antibodies.

Analytical and bioanalytical chemistry·2026

Same author

Assessment of Particle Size-Resolved Surface-Charge Density Distributions of Polymer Nanoparticles by Capillary Zone Electrophoresis.

Analytical chemistry·2025

Same author

Nephelometry as a simple tool to monitor the disassembly of polymeric nanoparticles.

Analytical methods : advancing methods and applications·2025

Same author

Quantification of Ceftazidime in the Vitreous Humor Using Ultra-performance Convergence Chromatography-Tandem Mass Spectrometry.

Therapeutic drug monitoring·2025

Search research articles

Related Experiment Video

Updated: May 27, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Developments in coupled solid-phase extraction-capillary electrophoresis 2009-2011.

Rawi Ramautar¹, Gerhardus J de Jong, Govert W Somsen

¹Biomolecular Analysis, Department of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. R.Ramautar@lumc.nl

Electrophoresis

|November 30, 2011

Summary

This review updates the literature on coupled solid-phase extraction-capillary electrophoresis (SPE-CE) systems from 2009-2011. It highlights advancements like carbon nanotubes and magnetic particles for enhanced sample analysis.

More Related Videos

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Related Experiment Videos

Last Updated: May 27, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

Published on: October 1, 2016

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

Analytical Chemistry
Separation Science

Background:

This review updates previous literature surveys on coupled solid-phase extraction-capillary electrophoresis (SPE-CE) systems.
It covers publications from January 2009 to July 2011, focusing on advancements since 2009.

Purpose of the Study:

To provide a comprehensive overview of the design and applications of SPE-CE systems.
To highlight emerging technological developments in SPE-CE.
To present a table summarizing recent SPE-CE studies.

Main Methods:

Review of literature on SPE-CE systems published between January 2009 and July 2011.
Discussion of both in-line and on-line SPE-CE approaches.
Inclusion of emerging technologies like carbon nanotubes and magnetic particles for sample extraction.

Main Results:

Detailed overview of SPE-CE system designs and applications.
Examples of SPE-CE applicability in biomedical, pharmaceutical, environmental, and food analysis.
A comprehensive table of recent SPE-CE studies including sample type, sorbent, coupling, detection, and LOD.

Conclusions:

SPE-CE systems continue to evolve with new materials and approaches.
The technique demonstrates broad applicability across various analytical fields.
Future perspectives and potential advancements in SPE-CE are discussed.