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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

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Published on: March 24, 2012

Protein disulfide isomerase and host-pathogen interaction.

Beatriz S Stolf1, Ioannis Smyrnias, Lucia R Lopes

  • 1Department of Parasitology, University of São Paulo, São Paulo, SP, Brazil.

Thescientificworldjournal
|November 30, 2011
PubMed
Summary

Reactive oxygen species (ROS) regulate host-pathogen interactions by affecting pathogen entry and immune cell function. Protein disulfide isomerase (PDI) plays a key role in these redox-dependent processes, offering therapeutic targets.

Keywords:
HostNoxPDIendoplasmic reticulumparasitespathogenredox

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Immunological cells produce reactive oxygen species (ROS) to combat pathogens.
  • Emerging evidence highlights ROS and redox signals as crucial regulators of host-pathogen interactions.
  • ROS impact pathogen entry, phagocytosis, and phagolysosome function, influencing antigen processing.

Purpose of the Study:

  • To summarize the cellular association of host Protein Disulfide Isomerase (PDI) with pathogens.
  • To explore the potential roles of pathogen-derived PDIs in infection.
  • To elucidate the complex redox-dependent regulatory mechanisms in host-pathogen interactions.

Main Methods:

  • Literature review and synthesis of existing research on ROS, PDI, and host-pathogen interactions.
  • Analysis of PDI's role in protein redox modification, pathogen entry, and phagocytic processes.
  • Investigation of PDI's involvement in endoplasmic reticulum (ER) and cytosolic thiol-based redox pathways.

Main Results:

  • Host PDI is implicated in regulating pathogen entry via protein redox switches.
  • PDI influences phagocytic ROS production by NADPH oxidase and phagolysosome function.
  • PDI's role in protein trafficking and antigen processing within the cytosol is highlighted.

Conclusions:

  • Understanding the intricate roles of ROS and PDI in host-pathogen interactions is vital.
  • Host-pathogen PDI interactions offer insights into coevolutionary processes.
  • Targeting these redox-dependent pathways may lead to novel therapeutic strategies against infections.