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Published on: February 27, 2015
An on-line solid phase extraction procedure for the routine quantification of caspofungin by liquid
Fabian Kirchhoff1, Barbara Maier, Christina Rieger
1Institute of Clinical Chemistry, Hospital of the University of Munich, Munich, Germany. fabian.kirchhoff@med.uni-muenchen.de
Background:
Extensive sets of data are required to investigate the potential use of a therapeutic drug monitoring with individualization of dosage of the antimycotic compound caspofungin. The goal was to develop an improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for this aim.
Methods:
Following protein precipitation, on-line solid phase extraction was performed for sample preparation. As the internal standard compound the veterinary drug tylosin was used. A standard validation protocol was applied.
Results:
Good reproducibility and accuracy of the method were observed. On-line solid phase extraction resulted in a convenient work-flow and good robustness of the method.
Conclusions:
This improved LC-MS/MS method was found reliable and convenient. It can be suggested for further work on the clinical pharmacology of caspofungin in the setting of clinical research laboratories.
