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Published on: September 25, 2018
[Establishment of molecular beacon real-time PCR for detecting p53 gene single nucleotide polymorphism]
Nong Liao1, Jian-hua Gao, Wei-sen Zeng
1Department of Obstetrics and Gynecology, Southern Medical University, Guangzhou 510515, China. lnong0109@sina.com
Objective:
To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene.
Methods:
Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed with molecular beacon real-time PCR. The results of SNP typing were compared with the results of reverse dot hybridization and confirmed by direct DNA sequencing.
Results:
The goodness of fit of this method was 100% in comparison with direct DNA sequencing, higher than that of reverse dot hybridization.
Conclusion:
Molecular beacon real-time PCR is suitable for rapid clinical detection of SNPs in p53 gene.
