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Updated: May 27, 2026

Mouse Eye Enucleation for Remote High-throughput Phenotyping

Mouse Eye Enucleation for Remote High-throughput Phenotyping

Published on: November 19, 2011

Mouse eye enucleation for remote high-throughput phenotyping.

Vinit B Mahajan¹, Jessica M Skeie, Amir H Assefnia

¹Department of Ophthalmology and Visual Sciences, University of Iowa, USA.

Journal of Visualized Experiments : Jove

|December 1, 2011

Summary

This summary is machine-generated.

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This study presents a remote tissue acquisition method for mouse eyes, enabling efficient global phenotyping of genetic models for human ophthalmic diseases. This approach facilitates broader research into eye conditions using transgenic mice.

Area of Science:

Ophthalmology
Genetics
Translational Medicine

Background:

Mouse models are crucial for studying human ophthalmic diseases like macular degeneration and glaucoma.
Transgenic and knockout mice, often generated for non-ophthalmic research, offer potential for discovering new eye-related genotype-phenotype correlations.
Current methods for phenotyping mouse eyes are geographically limited, requiring on-site expertise and hindering efficient, cost-effective research.

Purpose of the Study:

To introduce a novel remote tissue-acquisition method for mouse eyes.
To enable large or small-scale surveys of transgenic mouse eyes for phenotyping.
To facilitate molecular and morphological analyses of eyes from mutant animals regardless of their location.

Main Methods:

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A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

Published on: October 6, 2014

Microdissection of the Rodent Eye

Microdissection of the Rodent Eye

Published on: April 21, 2023

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Last Updated: May 27, 2026

Mouse Eye Enucleation for Remote High-throughput Phenotyping

Mouse Eye Enucleation for Remote High-throughput Phenotyping

Published on: November 19, 2011

A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

Published on: October 6, 2014

Microdissection of the Rodent Eye

Microdissection of the Rodent Eye

Published on: April 21, 2023

Development of standardized procedures for remote tissue acquisition.

Utilizing video-based surgical skill transfer for enucleation techniques.

Implementing protocols for tissue fixation and shipping of whole mouse eyes (unfixed and perfusion-fixed).

Main Results:

Demonstration of techniques for enucleating and transferring mouse eyes.
Establishment of a feasible method for remote phenotyping of transgenic mouse eyes.
The described method allows any laboratory to collect and send eyes for analysis.

Conclusions:

The remote tissue acquisition method overcomes geographical barriers in mouse eye research.
This technique enhances the utility of transgenic mice as models for human ophthalmic diseases.
It supports broader and more efficient discovery of genotype-phenotype correlations in the eye.