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Development and certification of green tea-containing standard reference materials.

L C Sander1, M Bedner, M C Tims

  • 1Analytical Chemistry Division, National Institute of Standards and Technology (NIST), Gaithersburg, MD 20899-8392, USA. lane.sander@nist.gov

Analytical and Bioanalytical Chemistry
|December 1, 2011
PubMed
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The National Institute of Standards and Technology (NIST) released three green tea Standard Reference Materials (SRMs) for quality control. These SRMs are characterized for key compounds and toxic elements, aiding analytical method development and traceability.

Area of Science:

  • Analytical Chemistry
  • Food Science
  • Pharmacognosy

Background:

  • Green tea (Camellia sinensis) consumption is widespread, necessitating reliable analytical methods for quality assessment.
  • Standard Reference Materials (SRMs) are crucial for ensuring accuracy and comparability in chemical measurements.
  • Existing SRMs for botanical products often lack comprehensive characterization for multiple analytes.

Purpose of the Study:

  • To introduce and characterize a suite of three new green tea Standard Reference Materials (SRMs).
  • To provide certified and reference values for key bioactive compounds and toxic elements in green tea products.
  • To support the development, validation, and metrological traceability of analytical methods for green tea analysis.

Main Methods:

  • Characterization using up to five distinct analytical methods.

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  • Measurement of catechins, xanthine alkaloids (e.g., caffeine), and theanine.
  • Quantification of toxic elements in the green tea matrix.

    • Main Results:

    • NIST has issued three SRMs: Green Tea Leaves (SRM 3254), Green Tea Extract (SRM 3255), and a Green Tea-Containing Solid Oral Dosage Form (SRM 3256).
    • Certified and reference values were assigned for targeted constituents, including bioactive compounds and toxic elements.
    • Collaborative measurements were performed at NIST and partner laboratories to ensure robust characterization.

    Conclusions:

    • The newly issued green tea SRMs provide essential tools for analytical laboratories.
    • These materials facilitate the development and validation of accurate and reliable methods for green tea analysis.
    • The SRMs support claims of metrological traceability in the analysis of green tea and related products.