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Updated: May 27, 2026

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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
Published on: April 20, 2018
Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies
Vardis Ntoukakis1, Benjamin Schwessinger, Cécile Segonzac
1The Sainsbury Laboratory, Norwich NR4 7UH, United Kingdom.
The Plant Cell
|December 2, 2011
Abstract:
Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.

