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Related Experiment Videos

Early changes in nuclear proteins following axotomy.

A Buriani1, M J Savage, D W Burmeister

  • 1Department of Pharmacology, Columbia University College of Physicians and Surgeons, New York, New York.

Journal of Neurochemistry
|November 1, 1990
PubMed
Summary

Following nerve injury (axotomy), early changes in nuclear proteins were observed in Aplysia neurons. These protein synthesis alterations in the nucleus are the earliest detected after axotomy and may impact axon regeneration.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Protein synthesis changes are crucial for neuronal response to axotomy.
  • Axonally transported proteins synthesized at higher rates may aid axon regeneration.
  • Regulation of these protein synthesis changes often occurs at the nuclear level via transactivating proteins modulating gene transcription.

Purpose of the Study:

  • To investigate early changes in nuclear proteins following axotomy in Aplysia neurons.
  • To identify specific nuclear proteins whose synthesis is altered post-axotomy.

Main Methods:

  • Single nuclei were isolated from [35S]methionine-labeled giant R2 neurons of Aplysia.
  • Proteins were analyzed using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D SDS-PAGE).

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  • This method ensured analysis of only neuronal nuclear proteins, avoiding contamination.

    • Main Results:

    • Significant increases in two nuclear proteins (56 kDa and 41 kDa) were detected 5 hours after axotomy.
    • Significant decreases in two other nuclear proteins (77 kDa and 46 kDa) were also observed at the same time point.
    • These represent the earliest reported post-axotomy changes in [35S]methionine-labeled proteins.

    Conclusions:

    • Early alterations in specific nuclear proteins occur within 5 hours of axotomy in Aplysia neurons.
    • These nuclear protein changes precede other reported post-axotomy protein synthesis alterations.
    • The identified nuclear protein modifications are potential early events regulating neuronal response and axon regeneration.