Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<b>The butterfly genus <i>Laothus</i> (Lycaenidae), with the description of a new species and a morphology-based phylogenetic outline</b>.

Zootaxa·2026
Same author

Formation and Coherent Propagation of Femtosecond-Laser-Induced Periodic Surface Structures (LIPSS) in Fluorine-Doped Tin Oxide: Control, Potential Applications, and Challenges.

ACS applied materials & interfaces·2026
Same author

Pulsed Electric Field Ablation Reprograms Tumor Immunity and Stimulates Germinal Center Formation in Tertiary Lymphoid Structures in Patients with Non-Small Cell Lung Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Incident learning systems to improve patient safety in nuclear medicine across Europe: results of the MARLIN study.

European journal of nuclear medicine and molecular imaging·2026
Same author

Development of ovine hepatic organoids: a powerful <i>in vitro</i> platform to reduce the number of experimental animals used in metabolism and nutrition assays.

Frontiers in veterinary science·2026
Same author

Chalcone and <i>Trans</i>-Chalcone Induce Transcriptomic Changes in <i>Caenorhabditis elegans</i> Compatible with a Novel Cumulative Damage Mode of Action.

Molecules (Basel, Switzerland)·2026

Related Experiment Video

Updated: May 27, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

NRPSsp: non-ribosomal peptide synthase substrate predictor.

Carlos Prieto1, Carlos García-Estrada, Diego Lorenzana

  • 1Institute of Biotechnology of Leon, INBIOTEC, Parque Científico de León, 24006 León, Spain. carlos.prieto@unileon.es

Bioinformatics (Oxford, England)
|December 2, 2011
PubMed
Summary

A new database and predictor tool identify substrates for non-ribosomal peptide synthetases (NRPSs), aiding the discovery of valuable peptide compounds. This resource facilitates research in biotechnology and microbial natural product synthesis.

More Related Videos

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
06:50

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Published on: January 26, 2024

Related Experiment Videos

Last Updated: May 27, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
06:50

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Published on: January 26, 2024

Area of Science:

  • Biochemistry
  • Computational Biology
  • Molecular Biology

Background:

  • Non-ribosomal peptide synthetases (NRPSs) are crucial enzymes in bacteria and fungi for producing diverse peptide compounds.
  • Individual domains within NRPSs exhibit specific substrate selectivity, influencing the final peptide products.
  • Discovering and characterizing novel non-ribosomal peptides is of significant interest to the biotechnology industry.

Purpose of the Study:

  • To develop a computational resource for identifying substrate specificities of NRPS modules.
  • To create a predictive model for substrate binding in NRPS systems.
  • To facilitate the discovery and biotechnological application of non-ribosomal peptides.

Main Methods:

  • Computational mining of existing data to build a comprehensive database of NRPS modules and their associated substrates.
  • Development of a hidden Markov model (HMM) based predictor utilizing the curated database.
  • Creation of a user-friendly website for accessing the database and predictor tool.

Main Results:

  • A database of NRPS modules with specific substrate binding information has been successfully constructed.
  • A hidden Markov model predictor capable of predicting substrate binding for given NRPS modules has been developed.
  • The database and predictor are publicly accessible via a dedicated website (www.nrpssp.com).

Conclusions:

  • The developed database and predictor represent a valuable tool for researchers in the field of NRPS.
  • This resource can accelerate the discovery and characterization of novel non-ribosomal peptides for biotechnological applications.
  • Computational approaches are effective in elucidating the functional specificities of complex enzyme systems like NRPS.