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Updated: May 27, 2026
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Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes
Published on: June 2, 2020
Isolation of primary avian osteocytes
Cor M Semeins1, Astrid D Bakker, Jenneke Klein-Nulend
1Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Amsterdam, The Netherlands. c.semeins@acta.nl
Methods in Molecular Biology (Clifton, N.J.)
|December 2, 2011
Abstract:
Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
