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Updated: May 27, 2026

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

Rodent osteoclast cultures.

Isabel R Orriss¹, Timothy R Arnett

¹Department of Cell and Developmental Biology, University College London, London, UK. i.orriss@ucl.ac.uk

Methods in Molecular Biology (Clifton, N.J.)

|December 2, 2011

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This chapter details methods for culturing rodent osteoclasts on dentine. These techniques enable independent study of osteoclast formation and function, focusing on extracellular pH

Area of Science:

Bone biology and cell culture techniques.

Background:

Osteoclasts are crucial for bone remodeling.
Understanding osteoclast function is key to bone disease research.

Purpose of the Study:

To describe quantitative methods for isolating and culturing rodent osteoclasts.
To enable independent study of osteoclast formation and activation.
To investigate the role of extracellular pH in osteoclast function.

Main Methods:

Quantitative isolation and culturing of rodent osteoclasts.
Utilizing dentine as a resorbable substrate.
Focus on controlling and measuring extracellular pH.

Main Results:

Generation of relatively large numbers of osteoclasts.

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Related Experiment Videos

Last Updated: May 27, 2026

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Published on: May 13, 2014

Ability to study osteoclast formation and activation independently.

Identification of extracellular pH as a critical factor.

Conclusions:

The described methods provide a robust system for osteoclast research.
Extracellular pH is a significant regulator of osteoclast activity.
This approach facilitates deeper understanding of bone resorption processes.