Jove
Visualize
Contact Us

Related Concept Videos

Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osteocyte Cell-Cell Communication Within and Beyond Bone.

Current osteoporosis reports·2026
Same author

Novel Three-Dimensional Preclinical Model for Investigating Cartilage Regeneration, Incorporating Physiological and Pathological Mechanical Loading.

Tissue engineering. Part C, Methods·2025
Same author

New lens on congenital mild bone fragility: a novel Col1a1 knockout mouse model for osteogenesis imperfecta type 1.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2025
Same author

Accuracy of CAD/CAM-based subtractive versus digital light processing fabricated mono-block splints for TMD management: An in vitro study.

Journal of dentistry·2025
Same author

Gingival fibroblasts produce paracrine signals that affect osteoclastogenesis in vitro.

Bone reports·2024
Same author

Developing and Investigating a Nanovibration Intervention for the Prevention/Reversal of Bone Loss Following Spinal Cord Injury.

ACS nano·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 27, 2026

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

Mechanical stimulation of bone cells using fluid flow.

Carmen Huesa1, Astrid D Bakker

  • 1Department of Developmental Biology, The Roslin Institute, University of Edinburgh, Edinburgh, UK. carmen.huesa@roslin.ed.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|December 2, 2011
PubMed
Summary
This summary is machine-generated.

This study details methods for applying fluid shear stress (FSS) to bone cells in vitro. It also covers measuring nitric oxide production in response to this mechanical stimulation.

More Related Videos

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes
05:03

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Published on: February 24, 2023

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

Related Experiment Videos

Last Updated: May 27, 2026

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
07:23

A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression

Published on: September 13, 2019

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes
05:03

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Published on: February 24, 2023

Mechanical Stimulation of Chondrocyte-agarose Hydrogels
12:45

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

Published on: October 27, 2012

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Mechanobiology

Background:

  • Mechanical stimuli, such as fluid shear stress (FSS), play a crucial role in bone cell function.
  • Understanding cellular responses to mechanical forces is vital for regenerative medicine and tissue engineering.

Purpose of the Study:

  • To describe established methods for applying controlled fluid shear stress (FSS) to bone cell monolayers in vitro.
  • To outline techniques for quantifying nitric oxide (NO) production as a response to FSS in bone cells.

Main Methods:

  • Utilizing a parallel-plate flow chamber to generate controlled fluid flow over cell monolayers.
  • Implementing a system for pumping culture medium through the chamber to create shear stress.
  • Employing methods to measure nitric oxide (NO) production in stimulated bone cells.

Main Results:

  • The described methods allow for reliable mechanical stimulation of bone cells.
  • Nitric oxide (NO) production can be effectively measured in response to FSS.

Conclusions:

  • The presented techniques provide a framework for investigating the effects of mechanical stimulation on bone cells.
  • These methods facilitate research into mechanotransduction pathways in bone biology.