Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The non-conventional peptidome of Arabidopsis flower development.

Plant physiology·2026

Same author

NextVir: Enabling classification of tumor-causing viruses with genomic foundation models.

PLoS computational biology·2025

Same author

The 'non-conventional' peptidome: A new layer in plant regulatory mechanisms.

Plant communications·2025

Same author

XVir: A Transformer-Based Architecture for Identifying Viral Reads from Cancer Samples.

Journal of computational biology : a journal of computational molecular cell biology·2025

Same author

Chronology of transcriptome and proteome expression during early Arabidopsis flower development.

Journal of experimental botany·2025

Same author

Inherent stochasticity, noise and limits of detection in continuous and time-gated fluorescence systems.

PloS one·2024

Search research articles

Related Experiment Video

Updated: May 27, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

FRET-based real-time DNA microarrays.

Arjang Hassibi¹, Haris Vikalo, José Luis Riechmann

¹Institute for Cellular and Molecular Biology, University of Texas, 1 University Station C8800, Austin, TX 78712-0323, USA. arjang@mail.utexas.edu

Methods in Molecular Biology (Clifton, N.J.)

|December 2, 2011

Summary

This summary is machine-generated.

This study introduces real-time DNA microarrays for enhanced quantification. The method uses hybridization kinetics to accurately measure analyte concentrations, improving upon conventional systems.

More Related Videos

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Related Experiment Videos

Last Updated: May 27, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Conventional DNA microarrays face limitations like probe saturation and variations affecting accurate quantification.
Existing methods struggle with a limited dynamic range and susceptibility to experimental artifacts.

Purpose of the Study:

To develop and validate a novel quantification method for affinity-based DNA microarrays.
To enhance the detection dynamic range and reliability of DNA microarray analysis.
To establish a real-time measurement approach for hybridization kinetics.

Main Methods:

Utilizing real-time measurements of DNA hybridization kinetics for quantification.
Developing a theoretical framework demonstrating the inverse relationship between target capturing time-constant and analyte concentration.
Implementing a Förster Resonance Energy Transfer (FRET)-based assay for experimental validation.

Main Results:

The real-time DNA microarray method overcomes limitations of conventional systems, including probe saturation and spot variations.
Demonstrated theoretical and practical inverse proportionality between target capturing time-constant and analyte concentration.
Successfully validated the method using a FRET-based assay for gene expression DNA microarrays.

Conclusions:

Real-time DNA microarrays offer a robust and enhanced dynamic range for analyte quantification.
The hybridization kinetics approach provides a reliable parameter for estimating analyte concentrations.
The FRET-based assay confirms the practical applicability of this real-time quantification method in gene expression studies.