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Updated: May 27, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
Published on: April 7, 2012
Metabolite analysis of Cannabis sativa L. by NMR spectroscopy
Isvett Josefina Flores-Sanchez1, Young Hae Choi, Robert Verpoorte
1Institute of Biological Chemistry, Washington State University, Pullman, WA, USA. i.flores.sanchez@chem.leidenuniv.nl
Abstract:
NMR-based metabolomics is an analytical platform, which has been used to classify and analyze Cannabis sativa L. cell suspension cultures and plants. Diverse groups of primary and secondary metabolites were identified by comparing NMR data with reference compounds and/or by structure elucidation using ¹H-NMR, J-resolved, ¹H-¹H COSY, and ¹H-¹³C HMBC spectroscopy. The direct extraction and the extraction by indirect fractionation are two suitable methods for the C. sativa sample preparation. Quantitative analyses could be performed without requiring fractionation or isolation procedures.
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