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Updated: May 27, 2026

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
Published on: December 12, 2013
Low voltage STEM imaging of multi-walled carbon nanotubes
Y Beyer1, R Beanland, P A Midgley
1Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK.
Abstract:
We present high magnification STEM images of multi-walled carbon nanotubes recorded with a 5 keV electron beam using a Helios Dual Beam microscope and a dedicated multi-segment transmission (STEM) detector. Images of carbon nanotubes recorded with bright-field (BF), annular dark-field (ADF) and high angle annular dark-field (HAADF) signals all show high contrast features, with internal structures 1-2 nm in width clearly revealed in the STEM images. Thicker regions of the nanotubes appear to show an unusual contrast reversal when comparing ADF and HAADF images. An understanding of the image contrast, and its dependence on thickness, is obtained by computing simulations of the ADF and HAADF images using Monte-Carlo software taking into account electron scattering in the nanotube.

