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Related Concept Videos

Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

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Related Experiment Video

Updated: May 27, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Abcg2 expression marks tissue-specific stem cells in multiple organs in a mouse progeny tracking model.

Soghra Fatima1, Sheng Zhou, Brian P Sorrentino

  • 1Division of Experimental Hematology, Department of Hematology, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Stem Cells (Dayton, Ohio)
|December 3, 2011
PubMed
Summary

The Abcg2 transporter identifies stem cells in bone marrow, liver, kidney, intestine, and testes. This study developed a new mouse model to trace Abcg2-expressing stem cells in various tissues, aiding stem cell research.

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Area of Science:

  • Stem cell biology
  • Molecular genetics
  • Hematopoiesis

Background:

  • The side population phenotype, linked to Abcg2 transporter activity, identifies hematopoietic stem cells (HSCs).
  • Abcg2 expression is a potential marker for adult stem cells in diverse organs.

Purpose of the Study:

  • To develop and utilize a lineage tracing mouse model to identify and track Abcg2-expressing stem cells.
  • To validate Abcg2 as a universal stem cell marker across multiple tissues.

Main Methods:

  • Generation of a novel mouse model co-expressing Abcg2 and CreERT2.
  • Crossing with lox-STOP-lox reporter lines (LacZ or YFP).
  • Tamoxifen-induced lineage tracing to identify Abcg2+ cells and their progeny.

Main Results:

  • Reporter gene expression confirmed physiologic Abcg2 patterns in liver and kidney.
  • Long-term marking of HSCs demonstrated their contribution to hematopoiesis.
  • Stem cell tracing observed in the small intestine and testes, confirming Abcg2 expression in their stem cells.
  • Labeling of interstitial muscle cells suggests a role in muscle repair.

Conclusions:

  • Abcg2 serves as a validated stem cell marker for blood, small intestine, and testicular germ cells.
  • Abcg2 may also mark stem cells involved in skeletal and cardiac muscle repair.
  • The developed model offers a novel, non-transplant-based approach for studying stem cell activity.