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Updated: May 27, 2026

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
Published on: February 22, 2019
The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence
Fany Reffuveille1, Pascale Serror2,3, Sylvie Chevalier4
1USC INRA 2017, Microbiologie de l'Environnement, EA956, Université de Caen, France.
Abstract:
Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn(2+) or under oxidative stress in vitro, and significantly decreased virulence.
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