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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 27, 2026

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
07:08

Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

Published on: October 20, 2020

Single-molecule gold-nanoparticle tracking.

Alexander R Dunn, James A Spudich

    Cold Spring Harbor Protocols
    |December 3, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Gold nanoparticle tracking offers high-resolution insights into molecular dynamics without external forces. This method enables submillisecond resolution for studying short-lived biological intermediates like myosin V.

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    Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

    Published on: July 15, 2014

    Related Experiment Videos

    Last Updated: May 27, 2026

    Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
    07:08

    Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison

    Published on: October 20, 2020

    Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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    Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles
    05:52

    Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

    Published on: July 15, 2014

    Area of Science:

    • Biophysics
    • Nanotechnology
    • Molecular Biology

    Background:

    • Single-molecule localization requires precise tracking methods.
    • Optical traps can influence diffusive processes being studied.
    • Fluorophores offer localization but may have limitations in temporal resolution.

    Purpose of the Study:

    • To present gold nanoparticle tracking as a high-temporal-resolution method for single-molecule localization.
    • To highlight the advantages of gold nanoparticle labels over optical traps for studying diffusive processes.
    • To detail a protocol for implementing gold nanoparticle tracking experiments.

    Main Methods:

    • Utilizing gold nanoparticles as labels for single-molecule tracking.
    • Employing microscopy techniques for high-temporal-resolution data acquisition.
    • Developing protocols for flow cell construction, microscopy, and data analysis.

    Main Results:

    • Gold nanoparticle tracking achieves nanometer accuracy in locating single molecules.
    • The method provides submillisecond time resolution, surpassing typical fluorophore capabilities.
    • Demonstrated application in characterizing structural properties of short-lived intermediates, exemplified by myosin V.

    Conclusions:

    • Gold nanoparticle tracking is a powerful technique for studying molecular dynamics with high temporal resolution.
    • The absence of external force application makes it ideal for diffusive processes.
    • The protocol is generally applicable for characterizing transient biological structures.