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Related Experiment Video

Updated: May 27, 2026

Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection
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Coating particles with carbocyanine dyes.

Josh L Morgan, Daniel Kerschensteiner

    Cold Spring Harbor Protocols
    |December 3, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Ballistic labeling uses tiny particles to quickly label dispersed neurons for imaging. This method effectively labels nearby cells with carbocyanine dyes, aiding neural development studies.

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    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Accurate imaging of developing neurons requires distinguishing individual cells.
    • Ballistic labeling offers a method for labeling dispersed cells using carrier particles.

    Purpose of the Study:

    • To describe a protocol for coating tungsten particles with carbocyanine dyes for neural labeling.
    • To optimize ballistic labeling for near-surface cells in neural preparations.

    Main Methods:

    • Coating tungsten particles (approximately 1 µm) with carbocyanine dyes.
    • Utilizing a pressurized gun for ballistic delivery of labeled particles.
    • Applying the protocol to label ganglion cells in retinal flat mounts.

    Main Results:

    • Carbocyanine dyes are lipophilic and highly fluorescent within lipid bilayers, suitable for neuron labeling.
    • Ballistic labeling allows rapid labeling of multiple dispersed cells in live or fixed tissue.
    • The protocol is most effective for labeling neurons near the tissue surface due to pressure-related damage.

    Conclusions:

    • Ballistic labeling with carbocyanine dyes provides an efficient method for neuron visualization.
    • The described protocol is optimized for labeling superficial neural cells, such as retinal ganglion cells.
    • This technique facilitates the imaging and reconstruction of developing neural circuits.