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Lipemia interferences in routine clinical biochemical tests
1Laboratory of Clinical Biochemistry, University Hospital Miguel Servet, Zaragoza, Spain. mpcalmarza@salud.aragon.es
Biochemia Medica
|December 6, 2011
Summary
Lipemia significantly interferes with clinical chemistry tests like phosphorus and calcium. Ultracentrifugation effectively removes these lipid interferences, improving analytical accuracy for crucial biochemical parameters.
Area of Science:
- Clinical Chemistry
- Analytical Biochemistry
Background:
- Lipemic specimens present a persistent challenge in clinical chemistry.
- Lipid interferences can significantly affect biochemical test results.
Purpose of the Study:
- To investigate the impact of lipid removal via ultracentrifugation on routine biochemistry parameters.
- To assess the effectiveness of ultracentrifugation in mitigating lipemia-induced interferences.
Main Methods:
- Selected lipemic samples underwent ultracentrifugation.
- Biochemical tests were performed before and after lipid removal.
- A total of 110 lipemic samples were analyzed.
Main Results:
- Significant differences were observed in most parameters post-ultracentrifugation, except for total bilirubin, glucose, GGT, and AST.
- Clinically significant interferences were identified for phosphorus, creatinine, total protein, and calcium.
- Alanine aminotransferase (ALT) showed the largest difference (7.36%), while glucose showed the smallest (0.014%).
Conclusions:
- Lipemia causes clinically significant interferences in measurements of phosphorus, creatinine, total protein, and calcium.
- Ultracentrifugation is an effective method for removing these lipid-induced interferences.
- Improving accuracy of routine biochemistry tests is crucial for patient diagnosis.
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