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Updated: May 27, 2026
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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Initial cord blood unit volume affects mononuclear cell and CD34+ cell-processing efficiency in a non-linear fashion
Sandrine Meyer-Monard1, André Tichelli, Carolyn Troeger
1Basel University Hospital , Basel , Switzerland.
Cytotherapy
|December 6, 2011
Summary
Optimizing umbilical cord blood (UCB) processing is crucial for hematopoietic stem cell therapy. Unit gross volume significantly impacts cell recovery, with optimal efficiency observed between 110-150 mL, suggesting careful consideration for processing very small or large UCB units.
Area of Science:
- Hematology
- Stem Cell Biology
- Bioprocessing
Background:
- Umbilical cord blood (UCB) is a vital source of hematopoietic stem cells (HSCs).
- Initial UCB use was limited to pediatric hematopoietic reconstitution.
- Expanding UCB applications to adults necessitates higher cellularity and optimized processing.
Purpose of the Study:
- To investigate parameters affecting cell recovery during UCB volume reduction.
- To optimize processing efficiency for total nucleated cells (TNC), mononucleated cells (MNC), and CD34+ cells (CD34C).
Main Methods:
- Analysis of 1553 UCB units undergoing routine volume reduction.
- Utilized hydroxyethyl starch-induced sedimentation with an automated device.
- Assessed cell recovery in relation to unit volume and cell counts.
Main Results:
- Unit gross volume, not TNC count, significantly impacts processing efficiency (PEf) for TNC, MNC, and CD34C.
- PEf non-linearly correlates with unit volume, peaking between 110-150 mL.
- Units < 90 mL and > 170 mL showed similarly poor PEf.
Conclusions:
- Unit gross volume is a critical factor influencing UCB processing efficiency.
- Fractionation of large UCB units should only be considered if resulting volumes exceed 90 mL.
- These findings aid in optimizing UCB processing protocols for enhanced HSC yield.
