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Allison L Zwingenberger1, William Vernau, Changying Shi

  • 1Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA. azwingen@ucdavis.edu

Leukemia Research
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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...