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Cloning embryos by nuclear transfer.

R S Prather1, N L First

  • 1Department of Meat and Animal Science, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.

Journal of Reproduction and Fertility. Supplement
|January 1, 1990
PubMed
Summary
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Nuclear transfer enables studying embryogenesis and creating identical individuals. Nuclear reprogramming in oocytes is key for development, but cell cycle asynchronies can limit success.

Area of Science:

  • Developmental biology
  • Cell biology
  • Reproductive biology

Background:

  • Nuclear transfer is a technique to study cell differentiation and produce cloned organisms.
  • Transferred nuclei undergo significant changes within the oocyte cytoplasm, including swelling and protein exchange.

Purpose of the Study:

  • To investigate the genomic modifications occurring after nuclear transfer.
  • To understand the limitations in development associated with differentiated donor nuclei.

Main Methods:

  • Analysis of nuclear changes (swelling, DNA synthesis, protein exchange) post-transfer.
  • Comparison of nuclear reprogramming efficiency in different species (amphibians, mammals).

Main Results:

  • Nuclear transfer induces swelling, DNA synthesis, and protein exchange, aiming for zygotic nucleus behavior.

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  • Developmental limitations are linked to cell cycle asynchronies and incomplete genomic reprogramming.
  • Incomplete DNA synthesis and reprogramming precede the first cell division.

    • Conclusions:

    • Genomic modifications are crucial for successful nuclear reprogramming after transfer.
    • Cell cycle asynchronies and insufficient reprogramming limit developmental potential.
    • Understanding these nuclear modifications is vital for improving cloning efficiency and developmental studies.