Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Depletion and replacement of tissue-resident macrophages in mice with germ-line deletion of a conserved enhancer in the Csf1r locus.

Development (Cambridge, England)·2026
Same author

LwaCas13a indiscriminately targets the human transcriptome.

Wellcome open research·2026
Same author

Selective vulnerability of cerebral vasculature to <i>NOTCH3</i> variants in small vessel disease and rescue by phosphodiesterase-5 inhibitor.

Science advances·2026
Same author

Analog of prolactin-releasing peptide reduces body weight primarily through sustained fatty acid oxidation rather than hypophagia.

Cell metabolism·2026
Same author

Inflammatory responses following CRISPR modification of the nuclear localisation sequence in endogenous interleukin-1 alpha.

Disease models & mechanisms·2026
Same author

GSK-3β coordinates axonal microtubule organization through Shot and Tau.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: May 27, 2026

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics
10:24

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Quantitative measurement of single cell dynamics.

Denise Bakstad1, Antony Adamson, David G Spiller

  • 1The University of Manchester, Manchester, M13 9PT, UK.

Current Opinion in Biotechnology
|December 6, 2011
PubMed
Summary

Luminescent and fluorescent imaging assays track cellular processes. New tools are needed to apply these dynamic cell biology techniques in vivo for better understanding cell fate.

More Related Videos

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
07:27

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Published on: May 13, 2012

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

Related Experiment Videos

Last Updated: May 27, 2026

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics
10:24

Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics

Published on: March 18, 2021

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
07:27

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Published on: May 13, 2012

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

Area of Science:

  • Cell biology
  • Biophysics
  • Molecular imaging

Background:

  • Luminescent and fluorescent imaging assays have advanced single-cell analysis over 20 years.
  • These techniques monitor transcription and protein translocation dynamics.
  • Integrating these assays with mathematical analysis significantly impacts cell biology.

Purpose of the Study:

  • To highlight the need for novel tools and technologies.
  • To translate current in vitro assays to in vivo studies of cells and tissues.
  • To advance the understanding of cellular heterogeneity and cell fate.

Main Methods:

  • Development of luminescent and fluorescent imaging assays.
  • Application of mathematical analysis to imaging data.
  • Focus on in vivo studies requiring new technological advancements.

Main Results:

  • Significant impact of current assays on cell biology.
  • Identification of an urgent need for in vivo translation.
  • Anticipation of new tools and techniques.

Conclusions:

  • Advancements in imaging assays have revolutionized single-cell studies.
  • Translating these assays to in vivo models is crucial for future research.
  • New technologies will enhance understanding of signaling, transcriptional dynamics, cellular heterogeneity, and cell fate control.