Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 27, 2026

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration
06:51

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration

Published on: June 7, 2012

Methods for long-term 17β-estradiol administration to mice.

E Ingberg1, A Theodorsson, E Theodorsson

  • 1Department of Clinical and Experimental Medicine/Clinical Chemistry, Linkoping University, Linkoping SE-581 85, Sweden. edvin.ingberg@gmail.com

General and Comparative Endocrinology
|December 6, 2011
PubMed
Summary

This study compared 17β-estradiol administration methods in mice. Peroral and silastic capsule methods provide reliable, physiological 17β-estradiol levels, outperforming commercial pellets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Parity-related variation in cortisol concentrations in hair during pregnancy.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Salivary cortisol circadian rhythm in infants at psychosocial risk showed more variations than previous studies of healthy full-term infants.

Acta paediatrica (Oslo, Norway : 1992)·2017
Same author

Cortisol levels in hair are altered in irritable bowel syndrome - A case control study in primary care.

Journal of psychosomatic research·2017
Same author

Cardiovascular risk factors and TIA characteristics in 19,872 Swedish TIA patients.

Acta neurologica Scandinavica·2016
Same author

Treatment of spontaneous intracerebral haemorrhage in Glanzmann's thrombasthenia.

Haemophilia : the official journal of the World Federation of Hemophilia·2012
Same author

Substance P alterations in skin and brain of chronically stressed atopic-like mice.

Journal of the European Academy of Dermatology and Venereology : JEADV·2012

Area of Science:

  • Endocrinology and reproductive biology
  • Pharmacology and drug delivery systems
  • Animal models in biomedical research

Background:

  • 17β-estradiol is crucial for understanding biological mechanisms.
  • Long-term administration methods for 17β-estradiol in rodents require thorough evaluation.
  • Accurate dosing is essential as 17β-estradiol effects vary with concentration.

Purpose of the Study:

  • To assess and compare different long-term 17β-estradiol administration methods in mice.
  • To identify reliable and physiologically relevant delivery systems for 17β-estradiol research.
  • To evaluate novel peroral and established subcutaneous methods against commercial pellets.

Main Methods:

  • 169 ovariectomized female C57BL/6 mice received 17β-estradiol via subcutaneous pellets, silastic capsules, or a novel peroral method.

More Related Videos

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
08:53

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

Related Experiment Videos

Last Updated: May 27, 2026

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration
06:51

Ovariectomy and 17β-estradiol Replacement in Rats and Mice: A Visual Demonstration

Published on: June 7, 2012

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria
08:53

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

  • Control groups included ovariectomized and intact mice.
  • Serum 17β-estradiol levels were measured weekly for five weeks using radioimmunoassay.

    • Main Results:

    • The peroral method maintained steady, mostly physiological 17β-estradiol concentrations.
    • Silastic capsules produced predominantly physiological levels, with transient peaks up to threefold the range.
    • Commercial pellets resulted in supra-physiological initial concentrations (0.18 mg) or extremely high levels (0.72 mg).

    Conclusions:

    • Peroral and silastic capsule methods are reliable for 17β-estradiol administration in mice.
    • These methods offer superior control over serum concentrations compared to commercial pellets.
    • Validated administration regimens are critical for accurate 17β-estradiol research in rodent models.