Related Experiment Video
Updated: May 27, 2026
11:10
Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
Published on: February 28, 2011
Ion release from magnesium materials in physiological solutions under different oxygen tensions
Frank Feyerabend1, Heiko Drücker, Daniel Laipple
1Institute for Material Research, Department of Macromolecular Structure Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany. frank.feyerabend@hzg.de
Journal of Materials Science. Materials in Medicine
|December 6, 2011
Summary
Developing predictive in vitro methods for magnesium alloys is crucial. Cell culture conditions offer a promising approach to simulate physiological corrosion, aiding in the design of new degradable biomaterials.
Area of Science:
- Biomaterials Science
- Corrosion Engineering
- Cell Biology
Background:
- Magnesium alloys show promise as degradable biomaterials, but predictive in vitro corrosion testing methods are lacking.
- Existing methods do not accurately reflect physiological conditions, hindering the development of new magnesium alloys.
- Simulating physiological corrosion in vitro is essential for advancing magnesium biomaterial research.
Purpose of the Study:
- To investigate the influence of cell culture conditions on magnesium alloy corrosion.
- To analyze the effects of different solutions, protein addition, and oxygen availability on corrosion behavior.
- To evaluate corrosion of pure Mg, WE43, and E11 magnesium alloys with varying surface finishes.
Main Methods:
- Incubation of magnesium materials (pure Mg, WE43, E11) under various cell culture conditions.
- Monitoring oxygen content, pH, osmolality, and ion release in solutions.
- Analysis of corrosion products, primarily identifying magnesium carbonate.
Main Results:
- Magnesium corrosion reduced oxygen content in solutions.
- Proteins enhanced the influence of oxygen on pH, without affecting osmolality.
- Magnesium ion release was solution-dependent, initially enhanced by proteins, with delayed release of alloying elements.
Conclusions:
- Cell culture conditions can induce physiological corrosion relevant to magnesium biomaterials.
- The study proposes cell culture incubation as a preliminary step for physiological corrosion assessment.
- Findings provide insights for designing predictive in vitro methods for magnesium alloy development.
Related Concept Videos
01:29Oxygen Requirements and Growth Patterns
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
03:24Common Ion Effect
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide:
03:46Qualitative Analysis
For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
For instance, group IV...
01:19Ionic Strength: Effects on Chemical Equilibria
The addition of an inert ionic compound increases the solubility of a sparingly soluble salt. For example, adding potassium nitrate to a saturated solution of calcium sulfate significantly enhances the solubility of calcium sulfate. Le Châtelier's principle cannot predict this shift in the equilibrium. Instead, this could be explained in terms of changes in the effective concentration of the ions in solution in the presence of added inert salt.
In this solution, the primary cation—the calcium...
In this solution, the primary cation—the calcium...
01:27Acids, Bases and Neutralization Reactions
Acids and bases play several important roles in biology. The pH of a biological system can significantly impact the function of biological molecules, including enzymes, proteins, and nucleic acids. For example, enzymes have optimal pH ranges for their activity, and changes in pH can denature or alter their structure, affecting their function. Acids and bases also play a crucial role in cellular signaling and communication. The pH of the extracellular fluid around cells can influence the...
01:27Feedback Regulation of Calcium Concentration
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...