Related Experiment Video
Updated: May 27, 2026
10:27
Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
Published on: December 15, 2011
Serological tests in gluten sensitivity (nonceliac gluten intolerance)
Umberto Volta1, Francesco Tovoli, Ronny Cicola
1Department of Clinical Medicine, St Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy. umberto.volta@aosp.bo.it
Journal of Clinical Gastroenterology
|December 6, 2011
Summary
Gluten sensitivity (GS) shows positive IgG antigliadin antibodies (AGA) in over half of cases, unlike celiac disease markers. This study differentiates GS serology from celiac disease, aiding in diagnosis.
Area of Science:
- Gastroenterology and Immunology
- Clinical Diagnostics
- Autoimmune Disorders
Background:
- Gluten sensitivity (GS) is a recognized clinical entity within gluten-related disorders.
- Diagnostic markers for GS are currently lacking.
- Differentiating GS from celiac disease is crucial for accurate patient management.
Purpose of the Study:
- To define the serological profile of gluten sensitivity (GS).
- To compare the serological findings in GS with those in celiac disease (CD).
- To identify potential diagnostic markers for GS.
Main Methods:
- Retrospective analysis of sera from 78 GS patients and 80 celiac disease patients.
- Assessed immunoglobulin (Ig)G/IgA antigliadin antibodies (AGA).
- Tested for IgG deamidated gliadin peptide antibodies (DGP-AGA), IgA tissue transglutaminase antibodies (tTGA), and IgA endomysial antibodies (EmA).
Main Results:
- IgG AGA positive in 56.4% of GS patients (vs. 81.2% in CD).
- IgA AGA detected in 7.7% of GS patients (vs. 75% in CD) with lower activity.
- No GS patients positive for IgG DGP-AGA, IgA tTGA, or IgA EmA (vs. high positivity in CD).
Conclusions:
- GS serology is characterized by IgG AGA positivity in over half of cases.
- GS lacks specific celiac disease markers like EmA, tTGA, and DGP-AGA.
- Distinct serological patterns aid in differentiating GS from celiac disease.
Related Concept Videos
01:30Serum Laboratory Studies, Stool Test, Breath Test
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.