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Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695
Dae Won Sim1, Jung Hyun Song, Woo Cheol Lee
1Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju, Chungbuk, Republic of Korea.
Abstract:
HP0902 from Helicobacter pylori 26695 belongs to the cupin superfamily of proteins, which encompasses proteins with a great diversity in function. In this work, two types of recombinant HP0902 protein were crystallized: one with an N-terminal His(6) tag ((H6)HP0902) and the other with a C-terminal His(6) tag (HP0902(H6)). The (H6)HP0902 crystal diffracted to 1.40 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.5, b = 78.6, c = 41.4 Å. The HP0902(H6) crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2 and diffracted to 2.5 Å resolution, with unit-cell parameters a = b = 50.4, c = 142.0 Å.
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