Related Experiment Video
Updated: May 27, 2026

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
Published on: September 14, 2017
Human endothelial progenitor cells isolated from COPD patients are dysfunctional
1Respiratory Department, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road, Guangzhou 51008, Guangdong Province, People's Republic of China. liuxiaoran3192@163.com
Chronic obstructive pulmonary disease (COPD) impairs endothelial progenitor cell (EPC) function, reducing their ability to repair blood vessels. This dysfunction in EPCs may contribute to the high rates of cardiovascular disease seen in COPD patients.
Area of Science:
- Cardiovascular Research
- Pulmonary Medicine
- Cellular Biology
Background:
- Cardiovascular disease (CVD) is a major cause of death in patients with chronic obstructive pulmonary disease (COPD).
- Atherosclerosis is prevalent in COPD patients, and endothelial progenitor cells (EPCs) are crucial for vascular repair and preventing atherosclerosis.
Purpose of the Study:
- To investigate the role of EPCs in vascular repair in patients with COPD.
- To determine if COPD affects the repairing capacity of EPCs.
Main Methods:
- Isolated EPCs from 25 COPD patients and 16 controls using Ficoll density-gradient centrifugation.
- Assessed EPC function via Transwell Migratory Assay and evaluated adhesion to human umbilical vein endothelial cells (HUVECs).
- Quantified EPC incorporation at injured vascular sites in mice using fluorescence microscopy.
Main Results:
- COPD patients exhibited significantly decreased EPC cluster formation, cell migration, and CXCR4 expression.
- Reduced adhesion of late EPCs to HUVEC tubules was observed in COPD patients.
- Fewer VEGFR2-positive EPCs were incorporated into injured vascular sites in COPD patients compared to controls.
Conclusions:
- EPCs' vascular repair capacity is impaired in patients with COPD.
- This diminished EPC function may contribute to the altered vascular endothelium and increased cardiovascular risk in COPD.
More Related Videos
08:43Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
Published on: February 14, 2017
13:46Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood
Published on: April 13, 2012
Related Concept Videos
Chronic Obstructive Pulmonary Disease-II: Pathophysiology
Chronic Inflammation
Chronic Obstructive Pulmonary Disease II: Emphysema
Chronic Obstructive Pulmonary Disease-I: Introduction
Chronic Obstructive Pulmonary Disease
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
Chronic Obstructive Pulmonary Disease I: Introduction
COPD: Pathogenesis and Clinical Features
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...